Ovulation is the release of an egg from the ovary. The few days around ovulation are the most likely times for conception to occur. The LH-Card rapid test is specifically designed to detect the LH surge.
Luteinizing hormone in elevated quantities causes ovulation. During the menstrual cycle only a small amount of LH is made, but in the middle of the cycle LH briefly increases. This increase is called the “LH surge” and precedes ovulation. Conception is most likely to occur within thirty-six hours following the LH surge.
In the test procedure, the test card is partially immersed in a urine specimen and allowed to soak for a period of five minutes. Urine flows vertically through the mem-brane causing several immunochemical reactions. First, lyophilized anti-LH-antibody-dye-conjugate binds to free LH in the urine specimen forming an LH-antibody-dye-complex.
Next, anti-LH-antibody immobilized in the test zone reacts with the LH-antibody-dye-complex forming an antibody-antigen-dye-conjugate-sandwich in the test zone visible as a pink-rose band, the intensity of which reflects the amount of LH in the sample specimen. Finally, an immobilized reagent in the control zone captures a dye conjugate forming a pink-rose control band, which is calibrated to correspond to a 25 mIU LH/ml intensity relative to the test band. At five minutes the test is complete and the control band intensity and test band intensity are visually compared to determine the concentration of LH present in the urine sample.
A test band of equivalent or greater intensity than the control band is a positive result, which indicates LH is present in the test sample at or above 25 mIU/ml, and suggests that the LH surge is occurring. A test band of lesser intensity than the control band is considered a negative result, and indicates the LH concentration of the specimen is below 25 mIU/ml, a good indicator that the LH surge is not occurring.

Product Feature

–High Sensitivity: 98%
–High Specificity: 96%
–Ease-to-use
–Reliable results
–Longer shelf life
–Clear background

Ordering Information:
  • 1. Cassette Cat No.: AN1027C
  • 2. Dipstick Cat No.: AN1027S
Helpful Documents:
  • 1. Instruction for use
  • 2. MSDS