Rota-Adeno is a rapid, qualitative, chromatographic immunoassay which simultaneously detects rotaviruses and adenoviruses in stool samples.

Rotaviruses and adenoviruses are responsible for the majority of pediatric diarrhea cases.

Rotavirus is the primary causative agent of acute gastro-enteritis, especially in children less than 2 years old. Its discovery in 1973 and its association with infantile gastro-enteritis represented a very important advance in the study of gastro-enteritis not caused by acute bacterial infection. Rotavirus is transmitted by oral-faecal contact with an incubation period of 1-3 daysetc.

The Adenovirus is the second most common cause of viral gastro-enteritis in children (10-15%). This virus may also cause respiratory diseases and, depending on the serotype, also diarrhoea, conjunctivitis, cystitis,etc. At least 47 serotypes of adenovirus have been described, all sharing a common hexon antigen.Serotypes 40 and 41 are the ones associated with gastro-enteritis, whose main symptom is diarrhoea thatmay last between 9 and 12 days associated with temperature and vomits.

The test uses new homogenous immunochromatographic system with gold particules. It is a ready to use test which only needs a faecal sample dilution with the supplied ready to use dilution buffer. Specificity is ensured by using a monoclonal antibody conjugated with gold particules and directed against specific human genus-specific Rotavirus antigen or Adenovirus antigens. The immunochromatographic stick is coated with a polyclonal immunoreagent specific for genus-specific Adenovirus hexon antigens or rotaviurs VP6 antigen. Liquid sample and gold conjugate both migrate by capillarity and reach the first specific anti-Adenovirus polyclonal reagent. If Adenovirus is present in the sample, it is blocked and immunoreaction appears as a red-pink line. As sample still migrates, it reaches the second specific reagent line against Rotavirus,  then migrate to the non specific anti-mouse IgG which gives rise to a third red-pink line. This line indicates that the chromatography has been developed without hindrance. It appears also with negative samples.

Ordering Information:

• 1. Cassette Cat No.: AN1004C
• 2. Dipstick Cat No.: AN1004S

Helpful Documents:

• 1. Instruction for use
• 2. MSDS