Myoglobin is a low molecular weight, cytoplasmic serum protein. Due to its low molecular weight, myoglobin is released more rapidly when muscle cells are damaged than other markers. Serum concentration of myoglobin increases above the normal range as early as 1 hour after myocardial infarction, and peak in approximately 4 to 8 hours after onset. Therefore, myoglobin is better suited for the early diagnosis of acute myocardial infarction(AMI).

Myoglobin Rapid Test Card is a sandwich immunoassay. When serum sample is added to sample pad, it moves through the conjugate pad and mobilizes gold anti-myoglobin conjugate that is coated on the conjugate pad. The mixture moves along the membrane by capillary action and reacts with anti-myoglobin antibody that is coated on the test region. If myoglobin is present at levels of 80 ng/ml or greater, the result is the formation of a colored band in the test region. If there is no myoglobin in the sample, the area will remain colorless. The sample continues to move to the control area and forms a pink to purple color, indicating the test is working and the result is valid.

Either whole blood, plasma or serum may be used as samples for this procedure. For the whole blood or plasma procedure, collect blood in a tube containing heparin as the anticoagulant. If serum samples are to be used, collect blood in a tube without anticoagulant and allow to clot. Since cardiac proteins are relatively unstable, it is recommended that fresh samples be used as soon as possible to collect critical patient information. Heat inactivation of samples may lead to hemolysis or protein denaturation and therefore should be avoided. Whole blood samples should be tested within 4 hours of collection. If specimens are to be stored, the red blood cells should be removed. Whole blood samples should not be frozen. Plasma or serum samples may be refrigerated for 24 hours at 2-8 °C. If plasma or serum samples are to be stored for more than 24 hours, they should be stored frozen at -20 °C or below.

Ordering Information:

• 1. Cassette Cat No.: AN1007C
• 2. Dipstick Cat No.: AN1007S

Helpful Documents:

• 1. Instruction for use
• 2. MSDS